Thus, we analysed CDK-RB-E2F genes expression, including but not limited to AURKB, BUB1, CDCA2 and DLGAP5. Our findings confirmed previous reports showing that AURKB gene is frequently overexpressed in tumour samples of lung cancer patients, a key regulator of mitosis associated with poor prognosis (Yu et al. 2018), possibly reflecting the requirement for increased mitotic spindle genes expression for increased replication maintaining a tumorigenic phenotype (Al-Khafaji et al. 2017). This evidence concerns the gene RB1 and neoplasm.