Next, to analyze if KCNQ1OT1 functions as an oncogenic lncRNA in colorectal cancer by regulating HK2 expression, we transfected KCNQ1OT1-knockdown HCT116 cells with the pcDNA3.1-HK2 plasmid and obtained HK2 levels that were similar to parental HCT116 cells as analyzed by western blotting (Figure 5A). Here, HK2 is linked to colorectal cancer.