For example, confocal microscopy for FISH demonstrated that lncRNA NKILA exerts its critical function in cellular cytoplasm preventing NF-κB activation through stabilization of NF-κB/IκB complex by playing an essential role in turning off cancer-associated inflammation [68]; while ISH method applied to miRNAs allowed the identification of miR-375 downregulation as prognostic factor of esophageal squamous cell carcinoma [69]. Here, NFKB1 is linked to cancer.