Interestingly, we found that, only RAF-22W, but not p110, rescued the sphere formation (Fig. 6a, b), which suggests that, in these two aggressive human cancer cell lines, MiaPaCa2 and MDA-MB231, ICMT regulates mutant KRAS function to maintain TAZ level and cancer stemness/self-renewal mainly through the RAS–RAF signaling pathway. This evidence concerns the gene ICMT and cancer.