CD8A and cancer: In order to determine whether our approach can be employed in other intensively studied, hard-to-transfect or primary cell types, we co-transfected small (3 kb) and large (15 kb) vectors to measure transfection efficiencies and cell survival in diverse adherent and non-adherent cancer cell lines (Huh7 and HepG2 (liver), PC3 (prostate), MCF7 (breast), HEK293 (kidney), A549 (lung), SH-SY5Y (neuronal), HL-60 (leukaemia)) as well as peripheral blood mononuclear cells (PBMCs) and purified CD8 T cells (Fig. 2a).