Fibroblast-derived iPSCs were generated from two healthy individuals (CON1 and CON2), three FXS patients lacking FMRP (FXS1, FXS2 and FXS3) and one isogenic embryonic stem cell (FMR1+/y;FMR1−/y) pair where the FMR1 gene was deleted using CRISPR/Cas9-mediated genome editing [24]. Here, FMR1 is linked to fragile X syndrome.