IL2 and neoplasm: In order to evaluate the interaction of hADSCs-IL2 with neuroblastoma cells and confirm the ability of hADSCs-IL2 to suppress SH-SY5Y proliferation in co-culture, we decided to culture tumor cells and hADSCs on a thin layer of Matrigel matrix (BD Biosciences, San Jose, CA, USA), which is rich in extracellular matrix (ECM) components and allows three-dimensional cell culture to be simulated [85].