While multiple signaling molecules have been associated with H2S, gene network analysis of representative genes from these pathways indicated that these functional networks converge and behave in a coordinated manner, being largely up-regulated with ischemia, universally down-regulated with GYY4137 treatment post-MI, and having generally reduced expression with endogenous CSE gene deletion (expression of Mir21a, Nppa and the genes of the renin-angiotensin system going against this trend in CSE−/− mice). The gene discussed is REN; the disease is ischemia.