However, it should be noted that this Kex1 RSA-based IgM ELISA presented a higher sensitivity (from 68.0 to 70.8%) and specificity (from 61.8 to 75.0%) for PcP diagnosis compared with our previous Msg RSA-based IgM ELISA, with a moderately high positive diagnostic likelihood ratio (2.83) and low negative diagnostic likelihood ratio (0.39) (Table 1). This evidence concerns the gene CD40LG and pneumocystosis.