Taken together, these results suggest that NF-κB (p65) may involve in the proliferation regulation of HCC by targeting miR-141 which targets CUL3, and the activation of the FGFR4 signaling pathway induces nuclear NF-κB (p65) expression via ERK-mediated signaling transduction in HCC. The gene discussed is FGFR4; the disease is hepatocellular carcinoma.