To determine the pathophysiological significance of TRPV4/NOX4 axis in asthma, WT and TRPV4 KO mice were subjected to intranasal inhalation of either saline or D. farinae antigen (25 μg), three times a week for five weeks and analyzed immune cells in BAL fluid, goblet cells and NOX4 transcript expression in lung. The gene discussed is NOX4; the disease is asthma.