In order to determine whether FN-EDA and TLR4 are necessary for Ad5.TGFβ2-induced ocular hypertension phenotype, we tested wildtype C57BL/6J (n = 17), B6.TLR4−/− mice (n = 8), B6.EDA+/+ mice (8), B6.EDA−/− (n = 18), B6.EDA−/− /TLR4−/− (n = 23) and B6.EDA+/+/ TLR4−/− (n = 16) mice in our model system (Fig. 3A). This evidence concerns the gene TGFB2 and ocular hypertension.