We found that the levels of TNF-α, IL-1β, and IL-6 were significantly increased, however, the production of IL-12 was remarkably decreased in LXN−/− mice during DSS-induced colitis, suggesting TNF-α, IL-1β, IL-6 and IL-12 involved in DSS-induced colitis in LXN−/− mice. The gene discussed is TNF; the disease is colitis.