In our study, lipid synthesis-related pathways were significantly enriched in the pathway enrichment analysis (among the top 10 significantly enriched pathways, 4 pathways were related to lipid synthesis), and genes involved in lipid synthesis, including ACACB, CYP21A2, CHKA, SCD, were downregulated in the HE- group. This evidence concerns the gene CHKA and hereditary elliptocytosis.