PINK1 and Abnormal emotional state: Likewise, to determine the impact of oxidative stress induced by loss of PINK1 and by psychological distress on mitochondrial function and antioxidant response pathways in PBMCs derived from the same cohort of animals, we assessed the level of mitochondrial content (translocase of the outer membrane 20 expression, TOM20) and antioxidant markers (DJ-1, SOD1 and SOD2) by performing Western blot (WB) of lysates from PBMCs derived from WT and PINK1-KO rats that were untreated or exposed to psychological distress.