van der Zande et al. 36 have shown that no obviously toxic effects after animals feed with silica nanoparticles (size: 5–200 nm) 100 to 2500 mg/kg body weight were observed after 28 days exposure; however, some adverse health effects were observed after 84 days of exposure, including the increases in serum alanine aminotransferase level, lipid droplets, and periportal liver fibrosis. This evidence concerns the gene GPT and Hepatic fibrosis.