This process correlates with poor prognosis as it correlates with cancer aggressiveness and lower survival,232,233 particularly, high M3 receptor expression correlates with poor prognostic and tumor budding.234 In prostate cancer patients, high cholinergic nerve densities correlate with low recurrence-free survival.190 Vesicular acetylcholine transporter (VAChT) is the usual marker of parasympathetic neurogenesis and it is usually quantified by immunostaining,235 and its hypothetical pharmacological regulation could alter tumor growth. This evidence concerns the gene SLC18A3 and Familial prostate cancer.