In KEGG pathway annotation, the pathways were mainly RNA transport and protein processing in the endoplasmic reticulum (Figure 2A): Ras, Rap1, and phosphatidylinositol-3 kinase/protein kinase B (PI3K/Akt) signaling pathways, which were enriched in the control group compared to hepatitis and HCC groups (Figure 2C, 2E). Here, AKT1 is linked to hepatocellular carcinoma.