Analysis of AChR (BTX-positive structures) localization in samples prepared with the iDISCO method confirmed that the organization of NMJs in Pax7−/− teratomas was different as compared to those derived from Pax7+/+ ESCs, i.e., in Pax7−/− teratomas, NMJ organization was impaired and the amount of AChRs was reduced in comparison to Pax7+/+ teratomas (Fig. 6h, i; Additional Movie 1 [Pax7+/+] and Additional Movie 2 [Pax7−/−]). The gene discussed is PAX7; the disease is teratoma.