Guided by this hypothesis, our current study has demonstrated that RIN3 expression was significantly increased in neurons of APP/PS1 mice; RIN3 interacted with BIN1/CD2AP to regulate APP trafficking and processing; Increased levels of RIN3 significantly impacted endocytic trafficking process and APP cleavage, that may lead to neuronal degeneration in early AD pathogenesis. This evidence concerns the gene APP and Alzheimer disease.