Thus, the protein levels of total GSK 3β, phosphorylated GSK 3βser9, nuclear β-catenin, and cyclin D1, which are the key signaling molecules in the Wnt signaling pathway, were measured in HOS and MG63 osteosarcoma cells transfected with siRNA1-URG4, siRNA2-URG4, NC, and blank by WB. Here, URGCP is linked to osteosarcoma.