Using quantitative PCR, we observed that the level of CH25H mRNA was significantly upregulated in frontal cortical tissue of the AD brain (n = 14) compared to age-matched (non-AD) controls (n = 9, p < 0.05) (Fig. 1a, all subjects were age > 80 and both genders were included). The gene discussed is CH25H; the disease is Alzheimer disease.