In CSF, T-tau and NfL were highly elevated in symptomatic prion disease-positive controls (p = 2.6 × 10−10 for T-tau; p = 1.6 × 10−11 for NfL, 2-sided Kolmogorov-Smirnov test; Fig. 3a, b). This evidence concerns the gene NEFL and prion disease.