In our targeted NGS discovery effort on 8 (PDAC and PAC tumours) and subsequent validation in another additional 85 more tumour tissues, we identified frequent mutations in genes like TP53, KRAS, SMAD4, CTNNB1, and ERBB3. Though, we have performed 8 paired (PDAC and PAC) samples, for targeted NGS studies, but after 10,000 times simulation with randomly taken 8 samples from databases showed similar sets of genes mutated in the diseased samples. This evidence concerns the gene SMAD4 and neoplasm.