To further test whether emodin could inhibit tumor angiogenesis by reducing tumor cell secreted VEGFA, we mixed Matrigel with mouse breast cancer cells 4T1 in the absence or presence of emodin, which could induce SerRS expression and reduce VEGFA expression in 4T1 as well (Figure 2C-D), and transplanted the Matrigel plugs subcutaneously into mice (Figure 2E). Here, VEGFA is linked to breast carcinoma.