ASAH1 and Renal cyst: To detect whether primary cilia (which play a key role in the development of ADPKD renal cysts) are affected in the Ganab+/- mice, we examined the specific marker of primary cilia, acetylated tubulin (Ac-α-tubulin), and Western blot results showed that Ac-α-tubulin was expressed in both the wild-type group and the Ganab+/- group (Figure 3(a)), and the difference was not significant (P = 0.457) (Figure 3(b)).