MC3T3-E1 and breast cancer cells were used for the chemotactic migration experiments, the results showed that MCF-7 and ZR-75-30 cells overexpressing RUNX1 WT repressed migration of cancer cells towards MC3T3-E1 cells compared with control, and the overexpression of RUNX1 T207A showed a marked suppressive effect compared with RUNX1 WT, while the overexpression of RUNX1 T207D significantly promoted cancer cells migrate to MC3T3E1 cells (Figure 5A and 5B). This evidence concerns the gene RUNX1 and breast cancer.