Based on the above results, we suggested that RNF8 assembled K63-linked polyubiquitination on β-catenin and facilitated its nuclear translocation in colon cancer cells, which in turn enhanced the expression of Wnt/β-catenin target gene MYC, promoting the proliferation of colon cancer cells (Figure 5G). This evidence concerns the gene RNF8 and malignant colon neoplasm.