HCT116 and SW480 cells with stable silencing of RNF8 were performed for the CCK8 proliferation assay (Figure 5A, Figure S4A) and colony formation assay (Figure 5B, Figure S4B), which demonstrated that silencing RNF8 retarded the growth of colon cancer cells in vitro. Here, RNF8 is linked to malignant colon neoplasm.