To validate the effect of AS on the expression of the five key genes (ADCY1, ADCY5, ADCY8, cAMP and Rap1) in cAMP and Rap1 signal pathways in pulmonary fibrosis, we measured their expression at gene level and protein level by RT‐qPCR and ELISA experiments, respectively. The gene discussed is ADCY8; the disease is pulmonary fibrosis.