In our study, we found that lncRNA NR2F1‐AS1 was positively related to the vessel target CD31 and CD34 in breast cancer, and could enhance the tube formation ability of HUVECs in vitro, which hinted the potential role of lncRNA NR2F1‐AS1 in breast cancer angiogenesis. This evidence concerns the gene PECAM1 and breast carcinoma.