ABCB5 and melanoma: Nevertheless, all four melanoma cell lines (M10, M14, FO1, and Colo38) expressed all aforementioned genes and the melanoma tissue stem and differentiation markers TyrOH, MelanA/MART1, and ABCB5. These molecular assays allowed us to ensure the efficiency, specificity, and sensitivity of our amplification procedures, considering the expected poor cellular samples.