Interestingly, the inhibitory effect of L9Mc on primary melanoma tumor volume is associated with a significant decrease in Ki-67 proliferation marker expression (59.44 ± 13.83% of positive cells) and increase in cleaved caspase-3 apoptosis marker (220.54 ± 59.87% of positive cells) while in the control and L9Mc SCR groups, the Ki-67 expression of positive cells were 100% and 93.75 ± 24.87% and the cleaved caspase-3 marker were 100% and 100.33 ± 39.58% of positive cells, respectively (Figure 4D). This evidence concerns the gene CASP3 and melanoma.