The OS-related multivariate analysis results showed that the expression of 28 genes (ABCC1, ANXA7, APOB, ATG7, BAK1, CA9, CCNA2, CHD1L, CYP3A4, E2F1, EZH2, F2, G6PC, GMPS, GOT2, HDAC2, HPX, KPNA2, LAPTM4B, MAGEB3, MAPT, MPV17, NTF3, PPAT, SLC2A1, SLC38A1, SPP1, and TRPV1) was significantly associated with OS in HCC patients. This evidence concerns the gene SPP1 and hepatocellular carcinoma.