We further verified F2, GOT2, and TRPV1 by three independent expression profile microarray data for liver cancer acquired from the Oncomine database, and conducted the quantitative real-time polymerase chain reaction (qRT-PCR) in 20 pairs of HCC and adjacent tissues, and immunohistochemistry (IHC) staining in 90 pairs of HCC and its precancerous tissues. This evidence concerns the gene TRPV1 and liver cancer.