Withaferin A (WA) is a steroidal lactone isolated from winter cherry (Withania somnifera (L.)Dunal) that has an ability to reduce FANCA protein levels and downregulates HSP90 expression in MDA-MB-231, SUM-149, MCF-7 breast cancer cells, and U2OS osteosarcoma cells as a platform of DSB repair reporter assays which demonstrated the disrupted interaction between FANCA-HSP90. Here, FANCA is linked to osteosarcoma.