In brief, our study indicates that DIM can modulate AHR leading to decreased transcription activities of relative genes and weakened interaction with EGFR to effectively reverse EMT process of ESCC with rearrangement of cytoskeleton and regulation of related EMT markers as well as MMPs through repressing RhoA/ROCK1-mediated COX2/PGE2 pathway which is connected by NF-κB. The gene discussed is AHR; the disease is esophageal squamous cell carcinoma.