INS and Insulin resistance: F0 females on HFD showed markedly increased fasting glucose (F0c: 3.7 ± 0.1 mmol/L vs. F0m: 4.2 ± 0.2 mmol/L, p = 0.039), AUC during IPGTT (F0c: 18.9 ± 0.6 mmol/L/h vs. F0m: 23.1 ± 1.4 mmol/L/h, p = 0.011), plasma insulin (F0c: 0.2 ± 0.04 ng/mL vs. F0m: 1.0 ± 0.2 ng/mL, p = 0.003), and HOMA-IR (F0c: 0.7 ± 0.1 vs. F0m: 3.9 ± 0.8, p = 0.003) compared to chow-fed dams, indicating glucose intolerance and insulin resistance in the overfed dams.