RT-PCR analysis of the transcriptional expression of cell cycle genes showed a significantly increased expression of ccnA1, ccnB2, ccnD3, ccnE1, cdc25b, and E2F1 and a significantly decreased expression of ccnD2 in the Fhit-transfected versus non-transfected tumor cells (Figure 2D). This evidence concerns the gene E2F1 and neoplasm.