To investigate transcriptomic changes in NSCLC cells in response to splicing factor silencing, an RNA sequencing experiment was performed on A549 NSCLC cells transfected with a non-targeting control siRNA (siNT), an siRNA targeting SNRPD3 (siSNRPD3), or an siRNA targeting SF3B1 (siSF3B1). The gene discussed is SF3B1; the disease is non-small cell lung carcinoma.