TWIST1 and breast cancer: Herein, we observed that the treatment of breast cancer cell lines with NETs upregulates the expression of several pro-inflammatory genes, including IL-8 (CXCL8), IL6, IL-1β (IL1B), and CXCR1. Furthermore, bioinformatics tools and chromatin immunoprecipitation assays identified many NF-κB binding sites along with the promoters of SNAI1, SNAI2, ZEB2, and TWIST1 genes [59].