The combined use of either four evaluated thresholds for AMH > 7.20 ng/mL, FAI > 2.75 ng/mL, testosterone > 1.15 nmol/L, androstenedione > 11.45 ng/mL, and LH/FSH ratio > 1.23 showed diagnostic accuracy above 90% in PCOS predicting in adolescents. The gene discussed is PLOD1; the disease is polycystic ovary syndrome.