Therefore, in the present project, we had the following objectives: first, we sought to study circulating levels of cytokines and soluble TLR4 in a cohort of biopsy-proven NAFLD women with morbid obesity (MO) in relation to the hepatic expression of TLRs (TLR2, TLR4, and TLR9). This evidence concerns the gene TLR4 and metabolic dysfunction-associated steatotic liver disease.