Spontaneous pain was accompanied by erythema (redness) and swelling (Figure 4C), which occurred immediately after injection of Tf2 and also unexpectedly after Tf2[S14R], suggesting that this inflammatory response is not mediated via activation of NaV1.3 but instead through an additional target (paw thickness: control, 2.5 ± 0.2 mm; Tf2, 3.2 ± 0.1 mm; Tf2[S14R], 3.5 ± 0.1 mm; p < 0.05; Figure 4D). Here, SCN3A is linked to Erythema.