CD274+ (or PDL1, gene for the PDCD1 ligand) and CD80+ (gene for CTLA-1 ligand) Tregs were significantly enriched in Tregs interacting with Texs while CD86+ Tregs were enriched in Tregs not interacting with Texs in CRC (Fig. 7g). This evidence concerns the gene CD86 and colorectal carcinoma.