GPT and Hepatic fibrosis: Additionally, we evaluated serum total bilirubin, hydroxyproline content, serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels and spleen weight/body weight ratio to provide evidence of the protective effect and anti-fibrotic properties of roseotoxin B in BDL-induced liver fibrosis (Fig. 1c).