ACTA1 and Hepatic fibrosis: As shown in Fig. 1c, the levels of ALT, AST and serum total bilirubin, the hydroxyproline content, and the spleen values were evidently decreased in mice with liver fibrosis following therapeutic treatment with roseotoxin B. The anti-fibrotic properties of roseotoxin B was further verified by Masson trichrome staining, Sirius Red staining, and α-SMA tissue expression (Fig. 2 and Supplementary Fig. 1).