We used several specific M2 markers (CD206, CD163, Arg1, and Fizz1) and M1 markers (CD11c, CD16/32, iNOS, and MHCII) for the study, as those markers have been used to distinguish M2/M1 TAMs in GBM xenograft models from our group18 and others55–57. Here, CD163 is linked to glioblastoma.