Further, since we aimed to administer the peptide orally to tumor-bearing flies, unlike in a previous study that involved tail vein injection of VGLL4-derived peptide (Jiao et al., 2014), we tagged the TONDU peptide with an HIV-TAT motif (RKKRRQRRR) and a nuclear localizing signal (NLS) (PKKKRKV) to facilitate cellular uptake (Wadia and Dowdy, 2005) and nuclear localization, respectively. This evidence concerns the gene VGLL4 and neoplasm.