LGALS4 and obesity due to melanocortin 4 receptor deficiency: Activation of different subpopulations of PAM-β’2 with eight distinct GAL4 transgenes (Aso and Rubin, 2016; Aso et al., 2014b) (MB056B, MB109B, MB042B, MB032B, MB312B, MB196B, MB316B, some of these also express in γ4) failed to rescue diet-induced obesity (Figure 4—figure supplements 1D and 2A).