To investigate the role of E2F6 in miR-454-mediated progression of ovarian cancer cells, pcDNA3.1-E2F6 plasmid was constructed and transfected separately or co-transfected with pCMV-MIR-miR-454 into OVCAR3 and SKOV3 cells (Fig. 5a). The gene discussed is E2F6; the disease is ovarian carcinoma.