WDFY3 and esophageal squamous cell carcinoma: To unveil the underlying biological roles of WDFY3‐AS2 in ESCC progression, three different siRNAs against WDFY3‐AS2 (WDFY3‐AS2 siRNA#1, #2 and #3) and a WDFY3‐AS2‐overexpressing plasmid (pcDNA3.1‐WDFY3‐AS2) were transfected into EC9706 and TE1 cells, and qRT‐PCR was utilized to confirm the transfection efficiency.