MiR‐2355‐5p significantly accelerated ESCC cell proliferation and invasion, whereas miR‐2355‐5p down‐regulation suppressed ESCC cell proliferation and invasion; however, these phenotypes were markedly reversed by WDFY3‐AS2 up‐regulation or down‐regulation. This evidence concerns the gene WDFY3 and esophageal squamous cell carcinoma.