These data were confirmed with application of either 2-ethyl-2-thiopseudourea (ETU) during ischaemia in WT mice (data not shown) or in eNOS−/− mice: all sham/non-anaemic eNOS−/− survived reperfused AMI, while five out of seven eNOS−/− with anaemia died. This evidence concerns the gene NOS3 and anemia (phenotype).