NOS3 and anemia (phenotype): These changes in LV function were accompanied by reshaping of the LV with increased sphericity (Fig. 1d–f) and a trend to increased eNOS expression (1.0 ± 0.4 versus 1.3 ± 0.6 eNOS normalised to housekeeping gene GAPDH, sham versus anaemia, n = 11/12, p = 0.25).