The redox imbalance in RBC from anaemic mice, in part driven by the uncoupling of RBC-eNOS, was further evidenced by slightly increased LVDP after treatment of anaemic RBC with the ROS inhibitor NAC (sham + NAC 35 ± 9 mmHg vs. anaemia + NAC 32 ± 6 mmHg). Here, NOS3 is linked to anemia (phenotype).